IN VITRO EFFECT OF RESORCINOL ON BOVINE SPERMATOZOA IN PROCESS OF CRYOPRESERVATION

Abstract

The purpose of present study was to observe the effect of resorcinol on bovine spermatozoa before and after cryopreservation. Fresh semen was obtained from six randomly chosen breeding bulls. Experimental samples were prepared by diluting the semen with six different concentrations of resorcinol (REZ1– 4; REZ2 – 2; REZ3 – 1; REZ4 – 0.5; REZ5 – 0.25 and REZ6 –0.152 mg.ml^-1). Experimental groups were compared against control group (REZK). Using CASA method spermatozoa motility was evaluated before cryopreservation and also after thawing, during incubation at 37°C in time periods 0, 5, 10, 15 and 60 minutes. Negative effect of in vitro resorcinol addition was observed in all analysed parameters (MOT, PRO, VCL, ALH and BCF) in groups REZ1, REZ2 and REZ3. In contrast, positive impact mainly on motility and progressive motility was found in experimental groups with the lowest resorcinol concentration addition (REZ4, REZ5 and REZ6) in comparison to the control group. After thawing significant effect of resorcinol on motility parameters were observed within each experimental group. Group with the highest concentration of resorcinol (REZ1) had markedly negative impact on evaluated motility parameters. Results of our study clearly confirm toxic effect of resorcinol on motility parameters of spermatozoa, which depend on concentration and time period.