EFFECT OF STORAGE TEMPERATURE ON THE MOTILITY CHARACTERISTICS OF ROOSTER SPERMATOZOA

Abstract

The objective of our study was to evaluate the influence of different storage temperature on rooster sperm motility. Semen was collected once a week from Lohmann Light breeder males into prepared sterile tube. The heterospermic pool was diluted at the ratio of 1:100 in a commercial avian extender, divided into two aliquots and incubated at 8°C or 37°C. The quality of semen samples were evaluated using CASA system (Sperm Vision™) after 30, 60 and 120 min of incubation. We observed significantly (P<0.05) the highest progressive motility of rooster sperm after 60 min of spermatozoa incubation at 8°C, that was also significantly (P<0.05) higher in comparison to spermatozoa incubated for 60 min at 37°C. Basing on the observed results, we hypothesized that law temperatures (about 8°C) would be better for long-term storage of rooster spermatozoa. Further experiments with different semen diluents and storage temperatures and intervals are required in order to prove our hypothesis.