EFFECT OF DIFFERENT DILUENTS ON ROOSTER SPERMATOZOA APOPTOSIS

Abstract

The aim of this study was to observe the apoptosis of rooster spermatozoa and to compare the effect of commercial insemination diluent and saline solution on rooster spermatozoa apoptosis. Semen samples were collected once a week from roosters lines Lohmann Light (n=30). The one heterospermic sample was diluted with saline solution at a ratio of 1:100 and the second was diluted with commercial insemination diluent (Avian diluents; IMV Technologies, France) in the same ratio 1:100 at room temperature. The percentage of apoptotic spermatozoa was estimated by fluorescent staining (Yo-Pro-1) 0.5, 1 and 2 hours after semen collection and analyzed by fluorescent microscopy. There were no significant differences in sperms’ membranes quality after half an hour and one hour between diluted semen in both heterospermic samples. Significantly higher rate (P<0.05) of Yo-Pro-1 – positive cells was observed in sample diluted in commercial diluents (22.03% vs. 35.91% of apoptotic spermatozoa) after two hours. We concluded that rooster semen could be diluted by saline solution which is cheaper and more accessible for practical The aim of this study was to observe the apoptosis of rooster spermatozoa and to compare the effect of commercial insemination diluent and saline solution on rooster spermatozoa apoptosis. Semen samples were collected once a week from roosters lines Lohmann Light (n=30). The one heterospermic sample was diluted with saline solution at a ratio of 1:100 and the second was diluted with commercial insemination diluent (Avian diluents; IMV Technologies, France) in the same ratio 1:100 at room temperature. The percentage of apoptotic spermatozoa was estimated by fluorescent staining (Yo-Pro-1) 0.5, 1 and 2 hours after semen collection and analyzed by fluorescent microscopy. There were no significant differences in sperms’ membranes quality after half an hour and one hour between diluted semen in both heterospermic samples. Significantly higher rate (P<0.05) of Yo-Pro-1 – positive cells was observed in sample diluted in commercial diluents (22.03% vs. 35.91% of apoptotic spermatozoa) after two hours. We concluded that rooster semen could be diluted by saline solution which is cheaper and more accessible for practical use.