RELATIONSHIP BETWEEN MOTILITY AND VIABILITY PARAMETERS OF FROZEN-THAWED BULL SPERMATOZOA

Abstract

The aim of this study was to determine relationship between parameters of spermatozoa motility (total motility - TM and progressive movement - PM) and viability of bull frozen-thawed spermatozoa (dead spermatozoa ratio, apoptotic spermatozoa ratio and plasma membrane integrity). Motility parameters were evaluated using computer-assisted semen analysis (CASA). Parameters of spermatozoa viability were analysed using fluorescent dyes PNA-FITC (plasma membrane), Yo-Pro-1 and propidium iodide (PI). All bulls (n=6) were divided into two groups. First group (n=3) A – better bulls with total motility after thawing over 40% and the second group (n=3) B – with total motility lower than 40%. It was observed significantly (P<0.001) higher TM and PM in group A. No significant differences in velocity parameters and ALH between the group A and B were detected. Occurrence of spermatozoa with disrupted membranes, dead/necrotic spermatozoa and apoptotic spermatozoa was significantly lower in the group A. Bulls in the group A showed significantly higher cleavage rate of embryos. These motility and viability characteristics are associated with a higher embryo cleavage rate in in vitro fertilizatioThe aim of this study was to determine relationship between parameters of spermatozoa motility (total motility - TM and progressive movement - PM) and viability of bull frozen-thawed spermatozoa (dead spermatozoa ratio, apoptotic spermatozoa ratio and plasma membrane integrity). Motility parameters were evaluated using computer-assisted semen analysis (CASA). Parameters of spermatozoa viability were analysed using fluorescent dyes PNA-FITC (plasma membrane), Yo-Pro-1 and propidium iodide (PI). All bulls (n=6) were divided into two groups. First group (n=3) A – better bulls with total motility after thawing over 40% and the second group (n=3) B – with total motility lower than 40%. It was observed significantly (P<0.001) higher TM and PM in group A. No significant differences in velocity parameters and ALH between the group A and B were detected. Occurrence of spermatozoa with disrupted membranes, dead/necrotic spermatozoa and apoptotic spermatozoa was significantly lower in the group A. Bulls in the group A showed significantly higher cleavage rate of embryos. These motility and viability characteristics are associated with a higher embryo cleavage rate in in vitro fertilization test.