THE THE VARIABILITY OF THE TOTAL POLYPHENOLS CONTENT AND THE ANTIOXIDANT ACTIVITY IN THE VARIETIES OF SELECTED LEGUMES

Abstract

The goal was to determine the variability of the total polyphenols content and the antioxidant activity in varieties of selected dry legume seeds, which were obtained from the soil of Piešťany. The soil in the Piešťany locality had a neutral soil reaction and was moderately humic. Of the risk elements, the total cadmium content exceeded the limit value by the Law No. 220/2004, but did not exceed the threshold value by the European Commission (2006). In the case of mobile forms of lead, the limit value by Law No. 220/2004 exceeded by 180%. The determination of the heavy metal content in legumes was performed using a flame AAS (instrument Varian AA Spectr DUO 240FS/240 Z/UltrAA). Based on the obtained results, we found the achievement of limit value of lead in 3 varieties of chickpea (Maškovský Bagovec, Kráľová from Krajová and Bušinský) and in 3 varieties of beans (Albena, Luna and Sina). For all 3 types of legumes, the difference between the individual varieties was statistically demonstrated when determining the total polyphenols content and the antioxidant activity values. Between species has been confirmed a statistically significant difference. The order of legumes based on the average decreasing content of total polyphenols is lentil>chickpea>bean and on antioxidant activity lentils>beans>chickpeas. The dependence of antioxidant activity on the total polyphenols has been confirmed in lentil varieties, in the case of chickpeas this relationship is statistically insignificant and a lower mean dependence was determined in beans.