THE CONTENT OF VITAMIN C AND ANTIOXIDANT ACTIVITY IN LESS-KNOWN TYPES OF FRUIT
Keywords:vitamin C, HPLC, antioxidant activity, DPPH, fruit
This study aims to determine the vitamin C content and antioxidant activity in different types of fruit: cornelian cherry (Cornus mas L.), service tree (Sorbus domestica), medlar (Mespilus germanica L.), pawpaw (Asimina triloba L.), persimmon (Diospyros kaki L.). The aim of the work was to compare these used methods. The fruit’s vitamin C content was determined by HPLC system Waters Separations Module 2695 with UV detector 2996. The content of vitamin C reached values from 72.238 µg. g-1 (Diospyros kaki L.) to 1114.61 µg.g-1 (Sorbus domestica). The antioxidant activity (DPPH) was determined spectrophotometrically. DPPH radical scavenging activity values were 2.28 (Mespilus germanica L.) – 12.34 μmol TE.g-1 DW (Cornus mas L.). Spearman's test showed a positive relationship between both monitored parameters. The content of vitamin C in the studied species showed a positive relationship with antioxidant activity. Due to the high content of vitamin C and antioxidant activity, future analyses should focus on the determination of other bioactive substances in these fruits.
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Copyright (c) 2022 Monika Ňorbová, Alena Vollmannová, Eduard Pintér, Marek Šnirc, Hana Franková, Silvia Fedorková, Natália Čeryová
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