USE OF TRAP MARKERS TO DETERMINE GENETIC RELATEDNESS IN NEW CASTOR BEAN (RICINUS COMMUNIS L.) LINES

Abstract

Castor bean (Ricinus communis L.) is a plant of immense importance worldwide and its main industrial use is for the production of castor oil. Knowing the diversity of castor is important in order to breed plants that will produce the most castor oil possible. In our work, we used a combination of 30 TRAP markers and 6 random primers to study genetic relatedness and similarity in a set of 111 new castor bean lines. Based on the results obtained with TRAP markers, we calculated the PIC value to determine genetic diversity. The 66 combinations of TRAP markers and 6 random primers we used together and determined 528 DNA bands that were present in all 111 castor bean lines analyzed. The lowest number of fragments (5) was determined using 7 combinations of TRAP markers and random primers (TRAP 04 ₓ arb 1, TRAP 19 ₓ arb 1, TRAP 40 ₓ arb 2, TRAP 53 ₓ arb 2, TRAP 22 ₓ arb 3, TRAP 30 ₓ arb 3 and TRAP 32 ₓ arb 4) and the highest (13) was determined using 2 combinations of primers (TRAP 37 ₓ arb 2 and TRAP 56 ₓ arb 2). The size of the amplified bands ranged from 200 bp to 2000 bp for all analyzed castor bean genotypes. In total, 360 polymorphic bands were determined, with an average value calculated as 5.45 polymorphic bands per primer. To determine the level of polymorphism, we calculated the PIC value, which determines the level of relatedness between the genotypes analyzed. Based on our results, we found and confirmed that combinations of TRAP markers and random primers are suitable for detecting castor bean polymorphism and can be used to improve ricin quality in further ricin breeding.