The PROTECTIVE EFFECT OF SELENIUM ON DIABETIC NEPHROPATHY IN WISTAR RATS

Authors

  • Samira Boussekine Laboratory of Bioactive Molecules and Applications, Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Larbi Tebessi University, Tebessa, Algeria
  • Sabrina Lekmine
  • Salim Gasmi University Larbi Tebessi Tebessa https://orcid.org/0000-0002-7682-2997
  • Abdelkarim Benkhedir
  • Hichem Saker
  • Abdelkrim Lidoughi Faculty of Medicine, University Badji Mokhtar, Annaba 23000, Algeria

DOI:

https://doi.org/10.55251/jmbfs.5960

Keywords:

Diabetes, Oxidative stress, Alloxan, Nephropathy, Selenium, Biochemical parameters

Abstract

The objective of the present paper is to study and evaluate the effect of selenium supplementation on diabetic nephropathy caused by reactive species generated following hyperglycemia.

This study conducted on thirty-five male albino Wistar rats divided into five groups : the first group serves as control (C), the second group was treated by intraperitoneal injection of selenium (sodium selenite ) at 1.89 mg/kg / day,  regarding the 3rd  groups diabetes was induced by intraperitoneal injection of 150 mg/kg of alloxan (single dose) (DM). One of the diabetic groups was treated by intraperitoneal injection of insulin, 3 IU/100 g PC twice a day (DTins).  Another diabetic group is treated with 1.89 mg/kg/day of sodium selenite intraperitoneally (DTSe), and this for 21 days (the two groups treated with selenium were previously treated by gavage of 2 mg/kg/day for 10 days by the same trace element).

The results showed in untreated diabetic rats, a drop in body weight, and an increase in blood glucose (13.87mmol/L ± 0.62), urea (10.58 mmol/l ± 0.59), creatinine (64 µmol/l ± 1.3), and phosphorus (1.8 mmol/l ± 0.2).  A decrease in serum calcium (1.9 mmol/l±0.1), total protein (58g/l ± 5), albumin (32 g/l ± 3), insulin (0.64 µ UI/ml ± 0.06) and enzymatic activity of serum G6PDH (109.8 m UI/10GR ± 3). With a decrease in anti-oxidant defense, which results in a reduced renal tissue content in GSH (55 nmol/mg of proteins ± 2.2) and of GPx (0.198 µmol/mg of P ± 0.007) and catalase (14.64 µmol H2O2/mn/mg of P ± 0.8) activities. Meanwhile an increase activity of GSTs (0.791 nmol/mn/mg of P ± 0.03) and the concentration of TBARS (0.382 nmol/mg of P ± 0.02) were observed.

Sodium selenite induced the antioxidant defense of renal tissue, which protected it from the free radical damage that can be caused by the injection of Alloxan.

Downloads

Download data is not yet available.

Downloads

Published

2022-08-01

How to Cite

Boussekine, S., Lekmine, S. ., Gasmi, S., Benkhedir, A. ., Saker, H. ., & Lidoughi, A. (2022). The PROTECTIVE EFFECT OF SELENIUM ON DIABETIC NEPHROPATHY IN WISTAR RATS. Journal of Microbiology, Biotechnology and Food Sciences, 12(1), e5960. https://doi.org/10.55251/jmbfs.5960

Issue

Section

Biotechnology