HINFI POLYMORPHISM OF PIT-1 GENE IN SLOVAK SPOTTED CATTLE

Abstract

In this study has been Pit-1 gene detected as the pituitary specific transcription factor that regulates the expression of the growth hormone and prolactin genes in the anterior pituitary. A total of 110 Slovak Spotted cows were genotyped for polymorphism of Pit-1/HinfI gene in exon 6 on bovine chromosome 1 by using polymerase chain reaction and restriction fragment length polymorphism methods. Digestion of PCR products with restriction enzyme HinfI revealed two alleles: allele A gave one fragments, 260 bp in length and allele B gave two fragments of 190 and 70 bp. The predominant allele was B with observed frequency 0.7045. In population were detected all three genotypes AA, AB and BB. The most frequent was heterozygous genotype AB with observed frequency 0.417. The population was in Hardy-Weinberg equilibrium.