OPTIMIZING CONDITIONS FOR SPECTROPHOTOMETRIC DETERMINATIONOF TOTAL POLYPHENOLS IN WINES USING FOLIN-CIOCALTEU REAGENT

Authors

  • Daniel Bajčan
  • ĽuboÅ¡ Harangozo
  • Diana Hrabovská
  • Dominika Bončíková

Keywords:

determination, Folin-Ciocalteu reagent, method, polyphenols, spectrophotometry, wine

Abstract

Wine is a complex beverage that obtains its properties mainly due to synergistic effect of alcohol, organic acids, arbohydrates, as well as the phenolic and aromatic substances. At present days, we can observe an increased interest in the study of polyphenols in wines that have antioxidant, antimicrobial, anti-inflammatory, anti-cancer and many other beneficial effects. Moderate and regular consumption of the red wine especially, with a high content of phenolic compounds, has a beneficial effect on human health. The aim of this work was to optimize conditions for spectrophotometric determination of total polyphenols in winwas to optimize conditions for spectrophotometric determination of total polyphenols in winwas to optimize conditions for spectrophotometric determination of total polyphenols in winwas to optimize conditions for pectrophotometric determination of total polyphenols in wine
using Folin-Ciocaulteu reagent. Based on several studies, in order to minimize chemical use and optimize analysis time, we have proposed a method for the determination of total
polyphenols using 0.25 ml Folin-Ciocaulteu reagent, 3 ml of 20% Na2CO3 solution and time of coloring complex 1.5 hour. We f

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Published

2013-02-01

How to Cite

Bajčan, D., Harangozo, ĽuboÅ¡, Hrabovská, D., & Bončíková, D. (2013). OPTIMIZING CONDITIONS FOR SPECTROPHOTOMETRIC DETERMINATIONOF TOTAL POLYPHENOLS IN WINES USING FOLIN-CIOCALTEU REAGENT. Journal of Microbiology, Biotechnology and Food Sciences, 2(special issue 2), 1271–1280. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7449

Issue

Vol. 2 No. special issue 2 (2013): Special issue 1 - part A

Section

Food Sciences

License

Copyright (c) 2013 Daniel Bajčan, ĽuboÅ¡ Harangozo, Diana Hrabovská, Dominika Bončíková

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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