EFFECT OF POLYMORPHISMS IN THE BOVINE GROWTH HORMONE GENE AND IMPACT OF INBREEDING DEPRESSION ON MILK PERFORMANCE TRAITS IN SLOVAK SPOTTED CATTLE

Authors

  • Eva Hazuchová
  • Nina Moravčíková
  • Ondrej Kadlečík
  • Anna Trakovická
  • Radovan Kasarda
  • Ivan Pavlík

Keywords:

cattle, growth hormone gene, inbreeding depression, milk production, PCR-RFLP, polymorphism

Abstract

The aim of this study was to evaluate the relationship between the polymorphism in the growth hormone (GH) gene, inbreeding and long-life milk performance traits in Slovak Spotted cows. Genetic diversity was evaluated based on pedigree analysis. DNA was isolated from 110 blood samples. The polymorphism of GH gene was genotyped using PCR – RFLP method. Digestion of PCR products with restriction enzyme AluI revealed two alleles L and V, with L as a dominant (0.6818). The most frequent genotype was LV with frequency 0.509. The differences in average long-life milk production traits by different GH genotypes were not significant. We found significantly higher the average long-life milk, protein and fat yield in outbreed animals with genotype LL and LV than in the groups of inbreed cows. Therefore, animals with heterozygote genotypes might have potentially positive effect on long-life milk performance traits.

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Published

2013-02-01

How to Cite

Hazuchová, E., Moravčíková, N., Kadlečík, O., Trakovická, A., Kasarda, R., & Pavlík, I. (2013). EFFECT OF POLYMORPHISMS IN THE BOVINE GROWTH HORMONE GENE AND IMPACT OF INBREEDING DEPRESSION ON MILK PERFORMANCE TRAITS IN SLOVAK SPOTTED CATTLE . Journal of Microbiology, Biotechnology and Food Sciences, 2(special issue 2), 1324–1334. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7463

Issue

Vol. 2 No. special issue 2 (2013): Special issue 1 - part A

Section

Biotechnology

License

Copyright (c) 2013 Eva Hazuchová, Nina Moravčíková, Ondrej Kadlečík, Anna Trakovická, Radovan Kasarda, Ivan Pavlík

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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