EVALUATION OF DOSE-DEPENDENT ACTIVITY OF BISPHENOL F ON VIABILITY PARAMETERS AND STEROIDOGENESIS IN H295R CELLS

Abstract

Increasing concern over bisphenol A (BPA) as an endocrine-disrupting chemical and recent imposition of restriction on the use of BPA paved the way for entry of its analogues in the market. Bisphenol F (BPF) is one of the major analogues of commercial value. Thus, its increasing production and application make it vulnerable to human exposure. The aim of our in vitro study was to assess the potential effect of BPF on H295R cells mitochondrial activity, metabolic activity, membrane integrity, lysosomal function, and testosterone synthesis. Adrenocortical carcinoma cells were cultivated during 24 h in the presence of BPF (0.1, 0.5, 1, 10, 25, 50, 75, 100, 300, 500 μM). Exposure doses of BPF caused a significant decrease of mitochondrial activity starting from 1 μM, we observed a slight increase in mitochondrial activity at the lowest concentration (0.1 μM). Metabolic activity decreased with increasing dose of BPF - from 10 to 500 μM. A significant increase in metabolic activity was observed after cultivation with 0.1 μM BPF and a slight increase was observed after cultivation with 0.5 μM BPF. We observed a slight increase in lysosomal function and membrane integrity after cultivation with 0.1 and 1 μM, although higher exposure doses (25 - 500 μM) caused significant decrease in membrane integrity and lysosomal function. Lowest exposure dose of BPF (0.1 μM) caused a significant increase in testosterone synthesis, on the other hand, higher exposure doses (50 - 500 μM) caused significant decrease of testosterone production. The obtained results confirmed that BPF at higher concentrations caused cytotoxicity and possibly have endocrine-disrupting potential.