THE EFFECT OF FRUCTOSE ON TURKEY SPERMATOZOA MOTILITY DURING IN VITRO CULTIVATION AT 41°C

Abstract

The aim of our study was to analyze the effect of different fructose concentrations: 20 mg.ml-1 – Y20/O20; 15 mg.ml-1 – Y15/O15; 10 mg.ml-1 – Y10/O10; 5 mg.ml-1 – Y5/O5 on the two age groups of turkey spermatozoa motility parameters (group Y – from 28 to 52 weeks and group O – from 53 to 68 weeks) after an in vitro cultivation at 41°C. Semen samples diluted with physiological solution were used as the control. Individual motility parameters were recorded at five time periods: 0, 1, 2, 3, 4 hours. Each sample was evaluated using the Computer Assisted Semen Analyzer (CASA) system. The selected spermatozoa motility parameters (MOT, PRO, VCL, ALH, BCF) were found to be balanced or significantly lower in all tested samples. After 1 hours of cultivation at temperature of cultivation (41 °C) significant decrease of all parameters was found. The evaluation of spermatozoa motility parameters between two different age groups turkeys detected higher values in group Y (Young; 28 – 52 weeks). The additions of fructose alone to the extender have not positive effect on turkey spermatozoa motility.