BACTERIA IN CHICKEN GASTROINTESTINAL TRACT DETECTED BY REAL TIME PCR AFTER BEE POLLEN APPLICATION IN CHICKENS DIET

Abstract

The aim of this study was to examine the effect of pollen on the microbial colonization of chicken gastrointestinal tract. The pollen was administered to both feed mixtures in various amounts except of the control group. The addition of 50 mg pollen to 1 kg of feed was included in the first experimental group, the addition of 100 mg.kg−1 in the second experimental group, the addition of 200 mg.kg−1 the third experimental group (EG), 300 mg kg−1 in the fourth EG and 400 mg kg−1 in fifth EG. The highest count of faecal enterococci was found in the fourth experimental group (EG) where 300 mg of pollen to 1 kg was added to feed mixture. The lower count of faecal enterococci was found in the first experimental group where 50 mg of pollen to 1 kg was added to feed mixture. The highest count of lactobacilli was found in the second and fifth experimental group where 100 g and 400 g of pollen to 1 kg were added to feed mixture. The lower count of lactobacilli was found in the first experimental group where 50 g of pollen to 1 kg was added to feed mixture.