OPTIMIZATION OF STANDARD SAMPLES DILUTION FOR DETERMINATION OF CRUSTACEANS BY THE PCR METHOD

Abstract

A current trend indicates the importance of protecting consumers by accurate product allergen labelling. Despite allergen labelling legislation consumers look for non-allergenic foods or products without trace allergen declaration rather than products including allergen. Therefore, food companies make an effort to minimize presence of allergens in products by effective cleaning in the production process so that the traces of allergens do not need to be declared on product labelling.A detection limit specified by optimalization of Real Time PCR. Reached value represents detection limit 10 ppm with quantitation range of reaction from 1 000 000 ppm to 10 ppm. The effectiveness of the method is 99.1% with a standard deviation of 2.27%. Reliability of the method determines the coefficient of determination 97.73% that is calculated based on the standard deviation of 0.0227. This method is suitable for precise designation of varieties of Family Crustaceae.