SCREENING OF ANTIMICROBIAL ACTIVITY AND GENES CODING POLYKETIDE SYNTHETASE AND NONRIBOSOMAL PEPTIDE SYNTHETASE OF ACTINOMYCETE ISOLATES

Authors

  • Silvia Kovácsová
  • Soňa Javoreková
  • Kamila Majerčíková
  • Juraj Medo

Keywords:

Actinomycetes, antimicrobial activity, PKS-I gene, NRPS gene

Abstract

The aim of this study was to observe antimicrobial activity using agar plate diffusion method and screening genes coding polyketide synthetase (PKS-I) and nonribosomal peptide synthetase (NRPS) from actinomycetes. A total of 105 actinomycete strains were isolated from arable soil. Antimicrobial activity was demonstrated at 54 strains against at least 1 of total 12 indicator organisms. Antifungal properties were recorded more often than antibacterial properties. The presence of PKS-I and NRPS genes were founded at 61 of total 105 strains. The number of strains with mentioned biosynthetic enzyme gene fragments matching the anticipated length were 19 (18%) and 50 (47%) respectively. Overall, five actinomycete strains carried all the biosynthetical genes, yet no antimicrobial activity was found against any of tested pathogens. On the other hand, twenty-one strains showed antimicrobial activity even though we were not able to amplify any of the PKS or NRPS genes from them. Combination of the two methods showed broad-spectrum antimicrobial activity of actinomycetes isolated from arable soil, which indicate that actinomycetes are valuable reservoirs of novel bioactive compounds.

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Published

2013-12-01

How to Cite

Kovácsová, S., Javoreková, S., Majerčíková, K., & Medo, J. (2013). SCREENING OF ANTIMICROBIAL ACTIVITY AND GENES CODING POLYKETIDE SYNTHETASE AND NONRIBOSOMAL PEPTIDE SYNTHETASE OF ACTINOMYCETE ISOLATES. Journal of Microbiology, Biotechnology and Food Sciences, 3(3), 255–258. Retrieved from https://office2.jmbfs.org/index.php/JMBFS/article/view/7044

Issue

Vol. 3 No. 3 (2013): December - January

Section

Microbiology

License

Copyright (c) 2013 Silvia Kovácsová, Soňa Javoreková, Kamila Majerčíková, Juraj Medo

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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