PROTEIN EXTRACTION OF MAIZE (ZEA MAYS) FOR PROTEOMIC 2 – DE ANALYSIS
DOI:
https://doi.org/10.15414/jmbfs.2014-15.4.3.263-265Keywords:
2 – DE, maize, sweet corn, total soluble proteinAbstract
A strength of two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is its ability to resolve and investigate the abundance of several thousand proteins in a single sample. Two different extraction procedures for two-dimensional electrophoresis of plant proteins are compared in this work. Phenol-based extraction methods have been mainly used to extract proteins from different organs or tissues on many species. We wanted to determine which of these protocols was optimal for starch plants in order to achieve both efficient protein extraction and high spot resolution on 2-D gels. The phenol-based protocol was superior to the sodium phosphatase methods, showing larger protein yields and greater spot resolution on 2-D gels.Downloads
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Published
2014-12-01
How to Cite
Gregová, E., KuÅ¥ka Hlozáková, T., Å liková, S., & Gálová, Z. (2014). PROTEIN EXTRACTION OF MAIZE (ZEA MAYS) FOR PROTEOMIC 2 – DE ANALYSIS. Journal of Microbiology, Biotechnology and Food Sciences, 4(3), 263–265. https://doi.org/10.15414/jmbfs.2014-15.4.3.263-265
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Section
Biotechnology
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Copyright (c) 2014 Edita Gregová, TÃÂmea KuÅ¥ka Hlozáková, Svetlana Å liková, Zdenka Gálová
This work is licensed under a Creative Commons Attribution 4.0 International License.
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