COMPARATIVE ANALYSIS OF Mal d 1.03A EXPRESSED TRANSCRIPTS IN PULP OF APPLE CULTIVARS GOLDEN AND SPARTAN

Abstract

Apples are one of the oldest, most desirable and most consumed fruit in the world and in our agroclimatic conditions apples are the fruit that are also in the winter mostly consumed fresh. As apples are available all the year, they are a very important source of secondary plant metabolites. On the other hand, apples do not provide only benefits and belong to the fruit that cause an allergic reaction in a sensitized the population. Mal d 1 protein, one of the apple allergens, belongs to the PR proteins and is encoded by multiple isoforms in the genome of Malus domestica Borkh. The aim of the study was to compare the level of expression of Mal d 1.03A isoform in the pulps of apple varieties Golden and Spartan during the ripening. Expression analysis of Mal d 1.03A allergen were performed in pulps from a total of six ripening stages ranged from the early up to the physiological maturity at approximately monthly intervals. Assessment of the level of amplification of transcripts of Mal d 1.03A allergen was performed by software comparing them to a well-defined value of 1000 ng × μl-1. In the comparing analysis, the levels of amplified transcripts of Mal d 1.03A were more balanced in the first stages of ripening in the variety Spartan. A high increasing of the amount of transcripts were obtained in the first stages of ripening in the variety Golden. In Continual increasing of Mal d 1.03A transcripts was observed the middle ripening stage for both varieties. At the end of the ripening a different situation was observed in analysed apple varieties. The expression of Mal d 1.03A allergen was balanced for the variety Golden but a high increase was observed at the end of the ripening for the variety Spartan.