IDENTIFICATION OF LIPOPEPTIDES PRODUCED BY BACILLUS AMYLOLIQUEFACIENS STRAINS
Keywords:Bacillus, lipopeptides, HPLC, MALDI-TOF/MS
In this study we isolated and identified the lipopeptides produced by Bacillus amyloliquefaciens strains 1/6k and 4/9, which were isolated from Adriatic Sea. Lipopeptides belong to the family of cyclic biosurfactants representing a group of surface active compounds produced by various microorganisms. Studied Bacillus spp. strains were able to produce cyclic lipopeptides surfactin, iturin A and fengycin. The characterisation of lipopeptides was studied by various analytical techniques (high-performance liquid chromatography â€“ HPLC and matrix-assisted laser desorption/ionization time-of-flight analysis for molecular mass determination â€“ MALDI-TOF/MS). These methods confirmed co-production of three lipopeptides â€“ surfactin, iturin A and fengycin by Bacillus amyloliquefaciens.
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Copyright (c) 2021 Zdenka BedloviÄovÃ¡, KarolÃna EnglerovÃ¡, RadomÃra NemcovÃ¡, Miroslava KaÄÃ¡niovÃ¡
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