IDENTIFICATION OF LIPOPEPTIDES PRODUCED BY BACILLUS AMYLOLIQUEFACIENS STRAINS

Authors

  • Zdenka Bedlovičová University of Veterinary Medicine and Pharmacy
  • Karolína Englerová University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice
  • Radomíra Nemcová University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice
  • Miroslava Kačániová Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia, Faculty of Biology and Agriculture, University of Rzeszow, Zelwerowicza St. 4, 35601 Rzeszow, Poland

DOI:

https://doi.org/10.15414/jmbfs.3690

Keywords:

Bacillus, lipopeptides, HPLC, MALDI-TOF/MS

Abstract

In this study we isolated and identified the lipopeptides produced by Bacillus amyloliquefaciens strains 1/6k and 4/9, which were isolated from Adriatic Sea. Lipopeptides belong to the family of cyclic biosurfactants representing a group of surface active compounds produced by various microorganisms. Studied Bacillus spp. strains were able to produce cyclic lipopeptides surfactin, iturin A and fengycin. The characterisation of lipopeptides was studied by various analytical techniques (high-performance liquid chromatography – HPLC and matrix-assisted laser desorption/ionization time-of-flight analysis for molecular mass determination – MALDI-TOF/MS). These methods confirmed co-production of three lipopeptides – surfactin, iturin A and fengycin by Bacillus amyloliquefaciens.

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Published

2021-04-01

How to Cite

Bedlovičová, Z., Englerová, K., Nemcová, R., & Kačániová, M. (2021). IDENTIFICATION OF LIPOPEPTIDES PRODUCED BY BACILLUS AMYLOLIQUEFACIENS STRAINS . Journal of Microbiology, Biotechnology and Food Sciences, 10(5), e3690. https://doi.org/10.15414/jmbfs.3690

Issue

Section

Microbiology
Received 2020-09-08
Accepted 2020-11-09
Online Published 2021-04-01

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